Loading an image
First load an image in Napari. The primary image formats supported are TIFs, PNGs, JPEGs/JPGs, BMPs, and GIFs.
There are two ways you can do this:
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You can drag and drop an image into Napari from file explorer or your desktop.
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You can use Napari’s toolbar to load an image:
- Click on “File.”
- Click on “Open File(s)…” from the context menu.
- Select an image from file explorer.
- Alternatively you can use ctrl + o to instantly bring up file explorer.
Ensure that the image you loaded is a 2D image. QuantPunc only supports 2D images.
You can check this by looking to see if there’s a scroll bar at the bottom of the GUI as displayed here:

If you see a scroll bar, that means you’ve uploaded a multi-layer image.
To split image stacks:
- Load your image stack as detailed above
- Right click on the layer you want to split.
- Click on “Split Stack” from the context menu.
- Repeat this process if you have an image stack that exceeds 3 dimensions.